HiTrap Capto S ImpAct Column, 5 × 5 mL (17371755) is a ready-to-use prepacked chromatography column set for screening and small-scale protein purification using ion exchange chromatography. Each pack contains five 5 mL HiTrap columns prepacked with Capto S ImpAct strong cation exchange resin for antibody polishing and method optimisation.
Key Features
- Ready-to-use 5 × 5 mL format, supplied as five prepacked HiTrap columns for small-scale purification work
- Capto S ImpAct resin, designed for efficient aggregate removal at high monoclonal antibody load
- Strong cation exchange selectivity, supporting ion exchange workflows for proteins and antibodies
- 10 to 500 mg antibody loading range, suited to screening and optimisation studies
- 5 mL bed volume, with 16 × 25 mm column dimensions in the HiTrap 5 mL format
- Practical flow-rate range, with 5 mL/min recommended flow and 20 mL/min maximum flow
- Regular-use pH range of 4 to 12, supporting broad method development conditions
- 50 µm particle size, supporting high-resolution intermediate purification and polishing workflows
Benefits
- Helps screen binding, loading, washing, and elution conditions before scaling to larger column formats.
- Supports small-scale purification workflows while keeping method development convenient and reproducible.
- Assists monoclonal antibody polishing where aggregate removal is a key process objective.
- Allows purification work to be run using a syringe, peristaltic pump, or chromatography system.
- Provides a useful 5 mL scale for bridging early screening and larger process development studies.
Why Choose the HiTrap Capto S ImpAct Column, 5 × 5 mL (17371755)?
The HiTrap Capto S ImpAct Column, 5 × 5 mL (17371755) is suited to laboratories developing strong cation exchange methods for mAbs, antibody variants, and other proteins. John Morris Group supplies Cytiva chromatography consumables to Australian laboratories with support for product selection and ordering.
