HiScreen Capto Butyl HIC Column, 4.7 mL (28992473) is a prepacked hydrophobic interaction chromatography column for small-scale screening and method development. It contains Capto Butyl resin in a 4.7 mL HiScreen format, supporting evaluation of low to medium hydrophobicity conditions in protein purification workflows.
Key Features
- 4.7 mL HiScreen prepacked format, with 0.77 × 10 cm column dimensions for small-scale chromatography development
- Capto Butyl HIC resin, intended for initial and intermediate purification steps requiring low to medium hydrophobicity
- Highly cross-linked spherical agarose matrix, with approximately 75 µm median particle size
- Butyl ligand chemistry, with approximately 53 µmol/mL resin ligand concentration
- Approximate 27 mg BSA/mL resin binding capacity, measured as dynamic binding capacity at 10% breakthrough
- Broad operating pH range, with operational stability from pH 3 to 13 and CIP stability from pH 2 to 14
- Defined HiScreen hardware pressure limit, rated at 8 bar (0.8 MPa, 116 psi)
Benefits
- Helps screen and optimise HIC conditions before moving to larger column formats.
- Provides butyl ligand selectivity where phenyl or octyl HIC media may not be the preferred choice.
- Supports method development while reducing the need to manually pack small screening columns.
- Can help reduce development time and sample use during resin and condition selection.
Why Choose the HiScreen Capto Butyl HIC Column, 4.7 mL (28992473)?
Choose the HiScreen Capto Butyl HIC Column, 4.7 mL (28992473) when you need a ready-to-use prepacked format for Capto Butyl HIC screening and optimisation. John Morris Group can help confirm availability and support selection of related Cytiva chromatography products for Australian laboratories.
