The HiScreen Capto Phenyl High Sub 4.7 mL Column (28992472) is a prepacked hydrophobic interaction chromatography column for method optimisation and parameter screening. The column is packed with Capto Phenyl (high sub) resin and has a 4.7 mL bed volume with a 7.7 mm x 100 mm bed size. It is designed for capture and intermediate purification work where medium to high hydrophobicity is required.
Key Features
- Prepacked Capto Phenyl high sub resin, supplied in a ready-to-use HiScreen column format
- 4.7 mL bed volume, helping reduce sample and buffer use during screening and optimisation
- 7.7 mm x 100 mm bed size, providing a 10 cm bed height for method development work
- Phenyl hydrophobic ligand, suited to HIC separations requiring medium to high hydrophobicity
- 75 µm median particle size, based on the d50v particle size listed for Capto Phenyl (high sub)
- 27 mg BSA dynamic binding capacity per mL resin, measured at 10% breakthrough
- 150 to 350 cm/h recommended operating flow velocity, with a maximum operating flow velocity of 600 cm/h
- Scalable process development format, supporting reproducible screening before transfer to larger formats packed with the same medium
Benefits
- Supports early HIC method development before moving to larger process-scale columns.
- Helps reduce buffer and sample consumption compared with larger screening formats.
- Allows resin selectivity and operating conditions to be compared in a compact prepacked column.
- Provides a practical format for capture and intermediate protein purification studies.
Why Choose the HiScreen Capto Phenyl High Sub 4.7 mL Column (28992472)?
The HiScreen Capto Phenyl High Sub 4.7 mL Column (28992472) is a useful choice when your lab needs a prepacked HIC column for screening medium to high hydrophobicity conditions. John Morris Group can assist Australian laboratories with Cytiva chromatography consumables and help confirm the right column format for your purification workflow.
