The Capto Butyl-S HIC Resin, 25 mL (17549701), is a hydrophobic interaction chromatography resin supplied as 25 mL bulk resin in 20% ethanol. It is designed for capture and intermediate stages of protein purification and is the least hydrophobic resin in the high-productivity Capto HIC resin range.
Key Features
- 25 mL bulk resin format, supplied in 20% ethanol for laboratory-scale column packing and method development
- Hydrophobic interaction chromatography resin, designed for capture and intermediate stages of protein purification
- Least hydrophobic Capto HIC option, supporting binding and elution of relatively strong hydrophobic molecules at comparatively low salt concentrations
- 1-butylmercaptan ligand chemistry, with a ligand concentration of 10.0-13.5 µmol/mL resin
- Highly cross-linked agarose matrix, supplied as spherical resin with an approximate 75 µm particle size
- High-flow pressure performance, with pressure/flow characteristics of ≥600 cm/h at <0.3 MPa in a 1 m diameter column with 20 cm bed height under stated test conditions
- Broad pH stability, with pH 3 to 13 operational stability and pH 2 to 14 cleaning-in-place stability
- Autoclavable resin format, specified for 17 min at 121 °C in 0.1 M KH2PO4, pH 8.0, for 10 cycles
Note: Capto Butyl-S is a custom designed resin produced on demand. Contact John Morris Group to confirm availability and lead time.
Benefits
- Supports purification of proteins and peptides where a lower-hydrophobicity HIC resin is preferred.
- Can help reduce denaturation risk for relatively strong hydrophobic solutes by enabling use at comparatively low salt concentrations.
- High-flow agarose matrix supports improved productivity and reduced process cycle time in suitable workflows.
- Bulk resin format allows laboratories to pack columns to suit development, screening and scale-up needs.
- Broad chemical and pH stability supports cleaning, sanitisation and robust process development.
Why Choose the Capto Butyl-S HIC Resin, 25 mL (17549701)?
Choose the Capto Butyl-S HIC Resin, 25 mL (17549701), when you need a lower-hydrophobicity Capto HIC resin for protein purification development, capture or intermediate purification. John Morris Group can supply this Capto chromatography resin for Australian laboratories working with hydrophobic interaction chromatography workflows.
