The HiScreen Capto Phenyl ImpRes Column, 1 x 4.7 mL (17548410) is a prepacked hydrophobic interaction chromatography column for method optimisation, parameter screening, and process development work. It is packed with Capto Phenyl ImpRes resin, using a highly cross-linked agarose matrix with a phenyl ligand for intermediate and polishing purification workflows.
Key Features
- Prepacked 4.7 mL HiScreen format, supplied as one column with a 10 cm bed height for method optimisation and screening
- Capto Phenyl ImpRes HIC resin, designed for intermediate and polishing steps where medium to high hydrophobicity is required
- Compact column geometry, with 0.77 x 10 cm column dimensions and an 8 bar hardware pressure limit
- High-resolution agarose matrix, using highly cross-linked spherical agarose with 40 µm median particle size
- Phenyl ligand chemistry, with approximately 9 µmol ligand density per mL of resin
- Verified binding capacity, with dynamic binding capacity of 19 mg BSA/mL resin at 10% breakthrough
- Broad operating window, with recommended operating flow velocity up to 220 cm/h and maximum operating flow velocity of 500 cm/h
- Wide pH stability range, with operational pH stability from 3 to 13 and cleaning-in-place stability from 2 to 14
Benefits
- Helps reduce sample and buffer consumption during early process development work.
- Supports convenient screening of HIC conditions before scale-up to larger formats.
- Enables high-resolution polishing when removal of contaminants is required.
- Provides scalable process data when used with the same resin and linear flow velocity in larger BioProcess columns.
- Offers a ready-to-use format that can be run on peristaltic pumps or chromatography systems such as ÄKTA systems.
Why Choose the HiScreen Capto Phenyl ImpRes Column, 1 x 4.7 mL (17548410)?
The HiScreen Capto Phenyl ImpRes Column, 1 x 4.7 mL (17548410) is suited to laboratories developing HIC polishing methods where resin selectivity, small-scale screening, and scalable conditions are important. John Morris Group supplies Cytiva prepacked chromatography columns to Australian laboratories and can assist with product selection and ordering.
