The Capto Phenyl ImpRes HIC Resin, 25 mL (17548401), is a hydrophobic interaction chromatography resin for high-resolution intermediate and polishing steps in downstream protein purification. It combines a high-flow agarose matrix with a 40 µm particle size and phenyl ligand chemistry. The 25 mL pack is suitable for resin screening, method development, and small-scale process optimisation.
Key Features
- High-resolution HIC resin, designed for intermediate and polishing purification steps
- High-flow agarose matrix, supporting strong pressure and flow performance in chromatography workflows
- Small 40 µm particle size, helping improve resolution compared with larger-particle HIC resins
- Phenyl ligand chemistry, providing hydrophobic interaction selectivity for protein purification
- Defined binding capacity, with 19 mg BSA/mL resin measured at 10% breakthrough under Cytiva test conditions
- Flexible operating window, with flow velocity up to 220 cm/h under specified column conditions
- Broad pH stability, with operational stability from pH 3 to 13 and short-term CIP stability from pH 2 to 14
- Strong chemical stability, suitable for commonly used aqueous buffers and standard CIP solutions including 1 M sodium hydroxide
Benefits
- Supports high-resolution separation during downstream purification method development.
- Helps optimise HIC conditions before moving to larger resin formats or prepacked process columns.
- Allows flexible process design through a wide operating range of flow velocities and bed heights.
- Can support improved productivity by enabling higher flow rates and practical column bed heights.
- Provides a chemically robust resin platform for repeated cleaning, storage, and development work.
Why Choose the Capto Phenyl ImpRes HIC Resin, 25 mL (17548401)?
The Capto Phenyl ImpRes HIC Resin, 25 mL (17548401), is a practical choice when high-resolution hydrophobic interaction chromatography is required for intermediate and polishing purification. John Morris Group supplies Cytiva chromatography resins to Australian laboratories with support for product selection and ordering.
