The Capto Phenyl High Sub HIC Resin, 25 mL (17545101) is a hydrophobic interaction chromatography resin for capture and intermediate stages of protein purification. It combines a highly cross-linked agarose matrix with a phenyl ligand, supporting high flow rates, useful binding capacity, and low back pressure in downstream purification workflows.
Key Features
- 25 mL bulk resin format, supplied as Capto Phenyl (high sub) resin for method development and protein purification workflows
- Hydrophobic interaction chromatography resin, used in capture and intermediate stages of protein purification
- Highly cross-linked agarose matrix, providing a spherical high-flow base for downstream chromatography
- Phenyl ligand chemistry, with a ligand concentration of approximately 27 µmol/mL resin
- Approximately 75 µm particle size, based on median particle size of the cumulative volume distribution
- Useful binding capacity, with QB10 dynamic binding capacity of approximately 27 mg BSA/mL resin under specified test conditions
- High pressure-flow performance, with at least 600 cm/h at up to 0.3 MPa in a 1 m diameter column with 20 cm bed height under stated conditions
- Broad pH stability, with operational pH stability from 3 to 13 and CIP pH stability from 2 to 14
Benefits
- Supports faster protein purification workflows by combining high flow rate, high capacity, and low back pressure.
- Helps reduce process cycle times during capture and intermediate purification stages.
- Provides a scalable HIC resin option for laboratories developing downstream protein purification processes.
- Supports cleaning and sanitisation workflows used in chromatography process development.
Why Choose the Capto Phenyl High Sub HIC Resin, 25 mL (17545101)?
The Capto Phenyl High Sub HIC Resin, 25 mL (17545101) is a suitable choice for laboratories selecting a medium to high hydrophobicity HIC resin for protein purification. John Morris Group supplies Cytiva chromatography resins to Australian laboratories, helping users source verified consumables for downstream purification and process development.
