The PreDictor RoboColumn CM Sepharose FF, 200 µL (29275282) is a miniaturised prepacked chromatography column for high-throughput process development. It is packed with CM Sepharose Fast Flow, a weak cation exchange resin based on cross-linked agarose for purification of a wide range of biomolecules.
Key Features
- Prepacked CM Sepharose Fast Flow minicolumn, using a weak cation exchange resin for biomolecule purification workflows
- 200 µL column volume, with a 10.0 mm bed height for small sample consumption
- Miniaturised RoboColumn format, designed for automated and parallel chromatographic separations
- Carboxymethyl ion exchange chemistry, with a CM ligand group on a cross-linked agarose matrix
- Defined ionic capacity, specified at 0.09 to 0.13 mmol/mL resin for CM Sepharose Fast Flow
- Approximately 90 µm particle size, specified as d50v for CM Sepharose Fast Flow
- Broad RoboColumn working range, with a working temperature of 4 °C to 30 °C and flow velocity of 16 to 1000 cm/h
- Array plate workflow, supplied in rows of eight columns that can be arranged on a 96-position array plate
Note: Avoid halogenated organic solvents and hexane with PreDictor RoboColumn units.
Benefits
- Helps reduce sample consumption during cation exchange resin and condition screening.
- Supports faster experimental setup by enabling automated, parallel chromatography workflows.
- Allows larger experimental spaces to be explored for improved process understanding.
- Can be used to screen binding, elution and wash conditions before moving to larger columns for verification.
- Provides a compact format for method development using CM Sepharose Fast Flow chemistry.
Why Choose the PreDictor RoboColumn CM Sepharose FF, 200 µL (29275282)?
Choose the PreDictor RoboColumn CM Sepharose FF, 200 µL (29275282) when you need a miniaturised prepacked weak cation exchange column for high-throughput purification method development. John Morris Group can supply this Cytiva consumable for Australian laboratories and help confirm suitability for your robotic liquid handling and chromatography workflow.
