PreDictor RoboColumn Capto Chelating, 600 µL (29275281) is a prepacked miniaturised chromatography column format for high-throughput process development. These minicolumns are packed with Capto Chelating, an immobilized metal ion affinity chromatography resin for purification of proteins with metal-ion affinity.
Key Features
- 600 µL PreDictor RoboColumn format, supplied as one row of eight columns with the same chromatography resin
- Prepacked with Capto Chelating resin, an IMAC resin for purification of native and histidine-tagged proteins
- Metal-ion charging flexibility, with Capto Chelating medium supplied free of metal ions for charging with a suitable ion before use
- Highly cross-linked agarose matrix, supporting chemical and physical stability in affinity chromatography workflows
- 30.0 mm bed height, with a 5 mm column internal diameter and 59 mm column height for the 600 µL format
- Polypropylene column and filter materials, with a filter pore size of 7 to 12 µm
- Automation-ready workflow format, intended for parallel experiments using robotic liquid handling systems
- Flexible operating window, with a working temperature of 4 °C to 30 °C and flow velocity range of 16 to 1000 cm/h
Benefits
- Helps laboratories screen IMAC purification conditions before transferring selected methods to larger chromatography columns.
- Supports high-throughput testing of binding, washing, elution and regeneration conditions with reduced sample consumption.
- Allows users to select an appropriate metal ion for the target protein and purification strategy.
- Reduces manual handling by supporting automated, parallel chromatography experiments in process development workflows.
Why Choose the PreDictor RoboColumn Capto Chelating, 600 µL (29275281)?
PreDictor RoboColumn Capto Chelating, 600 µL (29275281) is suited to laboratories developing IMAC methods for proteins with metal-ion affinity. John Morris Group can support Australian laboratories with Cytiva PreDictor RoboColumn units, chromatography consumables and process development supplies.
