The PreDictor RoboColumn Capto Chelating, 200 µL (29275280) is a miniaturised prepacked chromatography column for high-throughput process development. It is packed with Capto Chelating, an immobilized metal ion affinity chromatography resin for purification of proteins and peptides with affinity for chelated metal ions.
Key Features
- Prepacked Capto Chelating minicolumn, using an IMAC resin for purification of proteins and peptides with metal-ion affinity
- 200 µL column volume, with a 10.0 mm bed height for small sample consumption
- Miniaturised RoboColumn format, designed for automated and parallel chromatographic separations
- Highly cross-linked agarose matrix, with iminodiacetic acid groups coupled by stable ether linkages
- Defined Capto Chelating resin capacity, with a copper capacity of 23 to 33 µmol Cu2+/mL medium
- 75 µm resin particle size, specified as d50v for Capto Chelating medium
- Supplied free of metal ions, requiring charging with a selected metal ion before use
- Array plate workflow, supplied in rows of eight columns that can be arranged on a 96-position array plate
Note: Avoid halogenated organic solvents and hexane with PreDictor RoboColumn units.
Benefits
- Helps reduce sample consumption during IMAC resin and condition screening.
- Supports faster experimental setup by enabling automated, parallel chromatography workflows.
- Allows larger experimental spaces to be explored for improved process understanding.
- Provides a compact format for screening metal ion selection, binding, elution and wash conditions.
- Supports method development for proteins and peptides with affinity for chelated metal ions.
Why Choose the PreDictor RoboColumn Capto Chelating, 200 µL (29275280)?
Choose the PreDictor RoboColumn Capto Chelating, 200 µL (29275280) when you need a miniaturised prepacked IMAC column for high-throughput affinity purification method development. John Morris Group can supply this Cytiva consumable for Australian laboratories and help confirm suitability for your robotic liquid handling and chromatography workflow.
