PreDictor RoboColumn SP Sepharose FF, 8 × 200 µL (28986087) is a row of eight prepacked minicolumns for high-throughput ion exchange chromatography process development. Each 200 µL RoboColumn is packed with SP Sepharose Fast Flow, a strong cation exchange resin for fast protein purification.
Key Features
- Prepacked RoboColumn format, supplied as one row of eight 200 µL minicolumns for parallel chromatography screening
- SP Sepharose Fast Flow resin, a strong cation exchanger used for fast protein purification
- Sulphopropyl charged group, supporting strong cation exchange interactions across the resin operating range
- Miniaturised column volume, helping reduce sample and buffer consumption during process development
- 10 mm bed height, with a 5 mm column inner diameter and 39 mm column height
- Defined resin characteristics, with cross-linked 6% spherical agarose and approximately 90 µm particle size
- Strong cation exchange capacity, with 0.18 to 0.25 mmol H+/mL resin and approximately 70 mg ribonuclease A/mL resin binding capacity
- Broad RoboColumn operating range, with flow velocity from 16 to 1000 cm/h and maximum working pressure up to 8.0 bar
Benefits
- Supports high-throughput screening of cation exchange chromatography conditions in automated process development workflows.
- Parallel column format helps reduce hands-on time and experimental time compared with single-column screening.
- Small bed volume allows laboratories to explore more process conditions while reducing sample consumption.
- RoboColumn format can be arranged on a 96-position array plate for flexible automated workflow setup.
- SP Sepharose Fast Flow supports reliable method development before verification and scale-up to larger prepacked formats.
Why Choose the PreDictor RoboColumn SP Sepharose FF, 8 × 200 µL (28986087)?
PreDictor RoboColumn SP Sepharose FF, 8 × 200 µL (28986087) provides a compact, automated screening format for developing SP Sepharose Fast Flow cation exchange purification methods. John Morris Group can assist with Cytiva chromatography consumables and related high-throughput process development requirements for Australian laboratories.
