The HiTrap Capto MMC Column, 1 x 1 mL (29400465) is a prepacked multimodal ion exchange chromatography column for small scale protein purification. It is prepacked with Capto MMC, a multimodal BioProcess ion exchange resin designed for direct loading of clarified feedstocks and binding proteins at high or low ionic strength.
Key Features
- Prepacked 1 mL HiTrap column, supplied as one 1 x 1 mL column for convenient small scale purification and method development
- Multimodal weak cation exchange functionality, giving selectivity that differs from traditional ion exchangers
- Supports binding at high or low ionic strength, allowing proteins to bind in the presence of salt
- Designed for direct loading of clarified feedstocks, helping reduce dilution steps before sample application
- Optimised top filter design, supporting direct loading of sonicated unclarified cell lysate onto the column
- Compact 1 mL column bed, with a 7 mm diameter and 25 mm length
- Flow rate below 4 mL/min, with a maximum pressure rating of 5 bar
- High binding capacity at high conductivity, specified as greater than 45 mg BSA/mL medium
Benefits
- Helps laboratories screen selectivity, binding and elution conditions in a ready-to-use prepacked format.
- Supports method scouting for proteins that benefit from multimodal selectivity.
- Reduces preparation time compared with manually packing small chromatography columns.
- Provides a practical small scale format for evaluating Capto MMC resin before scale-up.
Why Choose the HiTrap Capto MMC Column, 1 x 1 mL (29400465)?
The HiTrap Capto MMC Column, 1 x 1 mL (29400465) gives laboratories a verified prepacked column option for multimodal ion exchange purification and direct clarified feedstock loading. John Morris Group can help confirm availability and support the selection of compatible chromatography consumables for your workflow.
