The HiScreen Capto Adhere Column, 1 x 4.7 mL (28926981) is a prepacked multimodal chromatography column for process development and parameter screening. The column is packed with Capto adhere resin in a 4.7 mL HiScreen format for monoclonal antibody polishing after Protein A capture.
Key Features
- Prepacked HiScreen column format, supplied as 1 x 4.7 mL for convenient process development workflows
- Capto adhere multimodal strong anion exchange resin, designed for post-Protein A purification of monoclonal antibodies
- 4.7 mL column volume, with 0.77 x 10 cm column dimensions and a 10 cm bed height for scalable experiments
- Highly cross-linked agarose matrix, with spherical particles and an approximate particle size of 75 µm
- Defined ionic capacity, specified at 0.09 to 0.12 mmol Cl-/mL resin
- Multimodal ligand selectivity, supporting ionic interaction, hydrogen bonding, and hydrophobic interaction mechanisms
- Recommended HiScreen sample loading conditions, with 150 to 300 cm/h flow velocity and 2 to 4 mL/min flow rate
- Biocompatible polypropylene column body, with a column hardware pressure limit of 0.8 MPa, 8 bar, 115 psi
Benefits
- Supports method optimisation and parameter screening for multimodal polishing workflows.
- Helps remove post-Protein A contaminants such as leached Protein A, aggregates, host cell proteins, nucleic acids, and viruses in flow-through mode.
- Helps reduce sample and buffer consumption while maintaining a process-relevant 10 cm bed height.
- Can be connected in series with a union when a 20 cm bed height is required for further method work.
- Provides a ready-to-use format for evaluating Capto adhere resin before scale-up to larger chromatography formats.
Why Choose the HiScreen Capto Adhere Column, 1 x 4.7 mL (28926981)?
The HiScreen Capto Adhere Column, 1 x 4.7 mL (28926981) is suited to laboratories developing and optimising multimodal polishing steps for monoclonal antibody purification. John Morris Group supplies Cytiva chromatography columns to support protein purification and process development workflows across Australian laboratories.
