Cole-Parmer Vector Laboratories EndTag 5' Nucleic Acid Labeling Kit provides an efficient and reliable system for labeling the 5’ ends of DNA, RNA, or unmodified oligonucleotides. Ideal for applications where internal labels may interfere with hybridization or sequence-specific protein binding, this kit enables rapid and uniform labeling in approximately one hour.
Product Features
- More efficient labeling of short oligonucleotides
- Simple—achieve reliable, uniform labeling in ~1 hour
- Diverse choice of labels: fluorophores, biotin, haptens, and affinity ligands (not included)
- Thiolate-specific chemistry enables covalent attachment of labels to nucleic acid ends
- Ideal for PCR primers as 3’ ends remain unmodified
- Kit performs 10 labeling reactions of up to 0.6 nmols of 5’ ends per reaction
Whats Included
- T4 polynucleotide kinase
- 10x reaction buffer
- ATPγS
- Precipitant
- Alkaline phosphatase
Benefits
- Efficient labeling without affecting hybridization or protein binding
- Rapid results in one hour for time-sensitive experiments
- Supports a variety of labeling reagents for flexible experimental design
Why Choose Cole-Parmer Vector Laboratories EndTag 5' Nucleic Acid Labeling Kit?
This kit provides a reliable and convenient solution for end-labeling nucleic acids, ensuring precise, reproducible results for molecular biology applications.
