ProTide Resins
Optimized PEG Resin Core with
Cl-TCP(Cl) and Cl-MPA Universal Linkers
ProTide™ resins contain an ideal PEG and polystyrene core leading to an ideal environment for the synthesis of difficult peptides with excellent swelling properties. New Cl-TCP(Cl) and Cl-MPA linkers incorporated onto ProTide eliminate the necessity for purchasing resins with pre-loaded C-terminal amino acids. The C-terminal amino acid reacts with the linker-chloride in the presence of potassium iodide (KI)1, N,N-diisopropylethylamine (DIEA), and microwave irradiation (Scheme 1). The process is automatically carried out on the Liberty Blue microwave peptide synthesizer, using pre- programmed methods in the software. The result, any amino acid can be loaded on the resins in 10 min.
Key Advantages:
- Automated, high-temperature loading procedure complete in 10 min, whereas room temperature takes up to 24 hours
- Avoids coupling reagents; therefore, eliminating epimerization and dipeptide formation that can occur during loading
- No longer need to buy/store > 20 different, pre-loaded acid-linked resins
- Exhibit strong stability towards hydrolysis during storage and handling
- TCP(Cl) is hyperacid sensitive and will produce protected peptides with 1% TFA/ DCM and further minimizes diketopiperazine2 and 3-(1-piperidinyl)alanine formation
1Sandhya K., Ravindranath B. Tetrahedron Lett. 49, 2435 2008
2Heinlein C., Silva D., Tröster A., Schmidt J., Gross A., Unverzagt C. Angew. Chem. 50, 6406 2011
The ultimate resin recommended for longer and more difficult sequences of peptide amides. Based on ideal swelling properties from a TentaGel® core, incorporating PEG PS with a loading of 0.15 – 0.25mmol/g.. This resin is unmatched for the routine synthesis of difficult peptides even > 75 amino acids.
