ProTide Resins

Optimized PEG Resin Core with 
Cl-TCP(Cl) and Cl-MPA Universal Linkers

ProTide™ resins contain an ideal PEG and polystyrene core leading to an ideal environment for the synthesis of difficult peptides with excellent swelling properties. New Cl-TCP(Cl) and Cl-MPA linkers incorporated onto ProTide eliminate the necessity for purchasing resins with pre-loaded C-terminal amino acids. The C-terminal amino acid reacts with the linker-chloride in the presence of potassium iodide (KI)¹, N,N-diisopropylethylamine (DIEA), and microwave irradiation (Scheme 1). The process is automatically carried out on the Liberty Blue microwave peptide synthesizer, using pre- programmed methods in the software. The result, any amino acid can be loaded on the resins in 10 min.

Key Advantages:

• Automated, high-temperature loading procedure complete in 10 min, whereas room temperature takes up to 24 hours
• Avoids coupling reagents; therefore, eliminating epimerization and dipeptide formation that can occur during loading
• No longer need to buy/store > 20 different, pre-loaded acid-linked resins
• Exhibit strong stability towards hydrolysis during storage and handling
• TCP(Cl) is hyperacid sensitive and will produce protected peptides with 1% TFA/ DCM and further minimizes diketopiperazine² and 3-(1-piperidinyl)alanine formation

¹Sandhya K., Ravindranath B. Tetrahedron Lett. 49, 2435 2008 
²Heinlein C., Silva D., Tröster A., Schmidt J., Gross A., Unverzagt C. Angew. Chem. 50, 6406 2011

N-Fmoc-leucine