Chlorolab 3+ system complete comprising:-DW3,OXYL1+,QRT1,LH36/2R,A2,S2/PL,S4,S10,S16_1215420
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Chlorolab 3+ system complete comprising:-DW3,OXYL1+,QRT1,LH36/2R,A2,S2/PL,S4,S10,S16

Overview

Chlorolab 3 System

Advanced system for the study of photosynthesis & respiration in Large Sample Volumes

  • Convenient, system for the advanced study of photosynthesis & respiration measurements in liquid-phase samples under illumination
  • DW3 oxygen electrode chamber for larger sample volumes up to 20 ml
  • Oxylab unit for direct PC control and data acquisition
  • Sample mixing by integral stirrer driving a magnetic follower
  • Illumination via LH36/2R red LED array via front optical port
  • Quantitherm light/temperature sensor for light calibration
  • System calibration & control via Windows® Software

Overview

Chlorolab 3 provides a sophisticated system for the advanced study of respiration and photosynthesis from larger liquid-phase sample volumes up to 20ml. The system is ideally suited to busy research facilities where demands on equipment performance are high but is equally at home in teaching environments for under & post-grad plant biology studies of the photosynthetic processes.

The DW3 is best suited to measurements of larger samples such as macroalgae or seaweed sections which can be suspended securely in liquid a medium of up to 20ml. DW3 is also suitable for suspensions of extracted chloroplasts, micro-algae etc when larger sample volumes are required. Changes in oxygen concentration of the sample medium are determined by the integral oxygen electrode mounted in the base of the chamber.

The system comprises the Oxylab control unit, S1 Clark type electrode disc, DW3 liquid-phase electrode chamber, LH36/2R red LED light source and QRT PAR/temperature sensor for light source calibration. All necessary accessories and spares are also included (excluding circulating water bath and PC).

OXYL1 – Oxylab Control Unit

The Oxylab oxygen electrode control unit operates in conjunction with the user-friendly O2view data acquisition and system control software to provide PC control of oxygen uptake or evolution measurements from the S1 Clark type oxygen electrode. Oxylab may be employed to function effectively across a broad range of applications from studies of mitochondrial and cellular respiration to measurements of isolated chloroplast suspensions or solid state leaf samples in photosynthesis research depending on the type of electrode chamber used.

Up to 2 individual Oxylab control units may be connected to a PC providing a convenient method of comparing signals from 2 channels simultaneously. Additionally, Oxylab may be configured to accept an optional auxiliary input signal (e.g. temperature, pH, chlorophyll fluorescence, TPP+ or other specific ion electrodes etc) using the appropriate accessory apparatus therefore extending the flexibility of the system.

Oxylab provides automation of complex light intensity changes during light response assays in conjunction with one of the compatible LED light sources available from Hansatech Instruments. Light tables (or photon flux density tables) are created within the O2view software via a user-friendly interface.

The Oxylab control unit connects to the serial port of a Windows® PC either directly or using a HAN/USB adapter (for newer PC’s with no serial ports). The control unit features a built-in magnetic stirrer (for liquid-phase measurements) and all the electronics required to control and measure the signal from the S1 oxygen electrode disc.

O2view Windows® software controls all major hardware and data acquisition functions including signal gain and back-off and simple calibration routines for both liquid and gas-phase measurements.

Data from the S1 electrode disc, optional auxiliary input signal and temperature signal from a QTP1 PAR/Temperature probe sensor are plotted as a chart recorder emulations in realtime with post-measurement data analysis tools included within the program. Completed experiments are saved in .CSV (Comma Separated Values) format which then may be opened directly in other Windows® data analysis applications such as Excel®.

DW3

The DW3 large volume electrode chamber is particularly suited to oxygen evolution / uptake measurements of macroalgae in seawater samples of between 1 – 20ml. (15-20ml if illumination is required). The square section borosilicate glass reaction vessel and quartz front window provides good optical qualities over a large surface area when illuminating the sample using the LH36/2R red LED light housing. Samples may either be in stirred suspension or in the case of laminar material, may be vertically supported and retained by the plunger assembly such that they may be fully illuminated.

A prepared S1 oxygen electrode disc is mounted beneath the reaction vessel and forms the floor of the vessel itself providing a highly sensitive and rapid response to small changes in oxygen tension within the sample. A rear optical window allows optional insertion of the fibre-optic cable from either the FMS 1 or FMS 2 pulse modulated chlorophyll fluorimeter (using DW3/FA adapter) to provide simultaneous fluorescence measurements.

S1 Electrode

The S1 electrode was designed in the early 1970’s during a collaborative project between Prof. David Walker, Prof. Tom Delieu and Hansatech Instruments. Since the first S1 oxygen electrode disc went on sale in 1974, the design and manufacturing process has remained largely unchanged in nearly 40 years – a true testament to the popularity, quality and reliability of the sensor.

A range of complementary instruments have been continuously developed over the years in order to meet the needs of emerging trends in the scientific world. Modern oxygen measurement systems incorporate the S1 electrode to provide sensitive and reliable tools for a vast range of applications from the demonstration of oxygen evolution from photosynthetic organisms to the analysis of mitochondrial respiration rates.

When prepared and fitted into an electrode chamber, the dome of the electrode disc forms the floor of the electrode chamber reaction vessel providing a sensitive and rapid response to small changes in oxygen tension within the sample.

The electrode disc comprises a central platinum cathode and a concentric silver anode. Preparation of the electrode includes the addition of electrolyte and the application of a thin oxygen permeable P.T.F.E. membrane to the electrode dome.

Once prepared and positioned in the electrode chamber, the disc is connected to an electrode control unit which applies a small polarising voltage between the platinum and silver electrodes. In the presence of oxygen, a small current is generated proportional to oxygen activity in the sample.

With modifications and in conjunction with accessory equipment, the S1 oxygen electrode disc may be used to measure other gasses such as hydrogen and nitric oxide.

Red LED Light Source (LH36/2R)

The LH36/2R consists of an array of 36 red LED’s and is designed for use with the DW3 liquid-phase electrode chamber and LD1/2 and LD2/3 gas-phase electrode chambers.

The LH36/2R requires a connection with the Oxylab electrode control unit which is responsible for the regulation of light intensity according to a user defined PFD (Photon Flux Density) table.

The LH36/2R has an integral cooling fan which automatically switches on to cool the housing when the light intensity reaches a certain point.

The LH36/2R has a maximum intensity of 750 µmol m-2 s-1 in the gas-phase chambers and 900 µmol m-2 s-1 in DW3.

Quantitherm PAR/Temperature Sensor

The QRT1 Quantitherm light meter and thermometer is specifically designed for use with the DW1, DW2/2 and DW3 liquid-phase oxygen electrode chambers.

Quantitherm overcomes the potential difficulties associated with accurately measuring PAR light levels and temperature within the reaction vessel of liquid-phase oxygen electrode chambers during photosynthetic experiments.

The temperature sensor operates across the range of normal measuring temperatures used for both photosynthesis research and cellular respiration studies and offers maximum accuracy in the 10°C – 40°C region.

For photosynthesis research, the PAR quantum sensor provides a displayed resolution of 1µmol m-2 s-1 throughout the 0 to 5,000 µmol m-2 s-1 range and additionally will measure extreme saturating light intensities (such as those used in pulse saturation techniques for chlorophyll fluorescence analysis) up to a maximum of 50,000 µmol m-2s-1 with a displayed resolution of 10 µmol m-2 s-1.

QTP1 Probe

The QTP1 probe sensor connects to the QRT1 control unit via a MiniDIN connection at the top of the control unit. The probe is designed to be mounted directly into the DW series electrode chambers via the use of suitable mounting collars (mounting collar for DW1 and DW2/2supplied, collar for DW3 supplied with DW3).

Despite being intended for light source calibration in liquid-phase oxygen electrode chambers, the PAR/temperature probe sensor must not be submerged in liquid. Although the probe is splash proof, prolonged contact with liquid will irreversibly damage the sensor. Calibration of light sources should be performed prior to the addition of samples. Damage caused by submersion will not be covered by warranty.

The probe provides both a PAR quantum sensor and a thermistor bead for temperature measurement and is constructed from stainless steel and acetal. Temperature is measured by an RT curve matched type glass bead thermistor mounted centrally in the probe tip. Photosynthetically Active Radiation (PAR) levels are determined by a quantum sensor located in the side wall of the probe.

The probe provides both a PAR quantum sensor and a thermistor bead for temperature measurement and is constructed from stainless steel and acetal. Temperature is measured by an RT curve matched type glass bead thermistor mounted centrally in the probe tip. Photosynthetically Active Radiation (PAR) levels are determined by a quantum sensor located in the side wall of the probe.

The QTP1 probe may also be connected directly to the rear of the Oxylab oxygen electrode control unit. O2view software plots the temperature signal from the QTP1 in real-time as a chart recorder emulation on the same screen as the signal from the S1 oxygen electrode disc. PAR values are also displayed in the O2view software light source calibration routine providing a convenient display of measured values during the 2-phase light source calibration process.

Accessories & Spares Supplied

  • A2 Membrane applicator
  • S2/PL Magnetic followers
  • S4 30m roll of PTFE membrane
  • S10 Spare O-rings for DW3 electrode chamber
  • S16 Electrode cleaning kit
  • HAN/USB RS232 – USB adapter

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