The ÄKTA pure 25 L Chromatography System (29018224) is a flexible liquid chromatography system for preparative protein, peptide and nucleic acid purification in research applications. This 25 L configuration provides fixed 280 nm UV monitoring, a system pump flow rate of 0.001 to 25 mL/min and a pressure range of 0 to 20 MPa.
Key Features
- Preparative ÄKTA pure 25 platform, designed for broad research purification tasks using flow rates up to 25 mL/min
- Fixed 280 nm UV monitoring, suited to routine purification of proteins and peptides containing aromatic amino acids
- High-pressure system pump, with 0.001 to 25 mL/min operation and up to 50 mL/min during column packing
- 20 MPa operating pressure range, equivalent to 200 bar or 2900 psi for compatible chromatography applications
- UNICORN software control, using UNICORN 7.6 or later for method control, run monitoring and result evaluation
- Included 1.4 mL mixer, with optional 0.6 mL and 5 mL mixer chambers available for workflow flexibility
- Gradient formation capability, with 0.1 to 25 mL/min gradient flow range and ± 0.6% gradient composition accuracy under stated conditions
- Rotary valve architecture, with a standard injection valve and optional inlet, outlet, column, loop, pH and versatile valve functions
- Conductivity and temperature monitoring, with 0.01 to 999.99 mS/cm conductivity reading range and 0°C to 99°C temperature reading range
- Modular system design, allowing hardware options such as sample pump, pH monitoring, fraction collection and additional valves to be configured for workflow needs
Benefits
- Supports routine research-scale purification while maintaining a compact footprint for laboratory bench or cold cabinet placement.
- Helps laboratories build reproducible protein purification methods using controlled flow, pressure, UV, conductivity and temperature data.
- Provides higher pressure capability than the ÄKTA pure 150 platform, making it suitable for compatible smaller-column workflows.
- Allows flexible method development across common chromatography workflows for proteins, peptides and nucleic acids.
- Can be expanded with optional modules as sample application, fraction collection or automation needs grow.
Why Choose the ÄKTA pure 25 L Chromatography System (29018224)?
The ÄKTA pure 25 L Chromatography System (29018224) is a strong choice for research laboratories needing flexible purification capability with fixed 280 nm UV detection and a 20 MPa system pressure range. John Morris Group supplies Cytiva chromatography systems across Australia and can help configure the system, accessories and software around your purification workflow.
