The Dissociation Enzyme Mix D, Kidney, 20 Reactions (29733434) is an enzyme mix kit for dissociation of kidney tissue in single-cell sample preparation workflows. Supplied as 1 pack × 20 reactions, it is developed for use with the VIA Extractor tissue disaggregator and supports cold enzymatic dissociation for downstream single-cell RNA sequencing applications.
Key Features
- Optimised for kidney tissue dissociation, supporting preparation of viable single-cell suspensions from kidney samples
- Developed for VIA Extractor workflows, enabling semi-automated tissue disaggregation using Cytiva's tissue disaggregator platform
- Two-enzyme and buffer kit composition, providing the core reagents required for the dissociation enzyme mix D workflow
- Cold dissociation operating temperature, with use at 4°C to help reduce cell stress during sample processing
- Defined VIA Extractor method settings, using 200 rpm for 15 min with a 2°C program temperature setting
- Sample amount guidance included, supporting up to 300 mg of tissue with 5 mL of enzyme mixture
- Sequential filtration workflow, using 100 µm, 70 µm and 40 µm cell strainers in the Cytiva method
- Demonstrated single-cell preparation performance, with Cytiva data showing cell viability greater than 80%, aggregation below 10% and yield over 2 × 107 cells/g when used with VIA Extractor
Benefits
- Helps prepare kidney tissue samples for single-cell RNA sequencing and related single-cell workflows.
- Supports gentler dissociation by using a controlled cold-temperature process.
- Reduces workflow development time by providing a tissue-specific enzyme mix and defined method parameters.
- Helps improve consistency when processing kidney samples using the VIA Extractor tissue disaggregator.
- Supports cleaner downstream analysis by helping reduce aggregation and improve viable single-cell recovery.
Why Choose the Dissociation Enzyme Mix D, Kidney, 20 Reactions (29733434)?
This dissociation enzyme mix D kit is a kidney-specific reagent pack for controlled tissue disaggregation in single-cell sample preparation. John Morris Group supplies Cytiva consumables across Australia, helping laboratories source verified reagents for tissue disaggregation, scRNA-seq preparation and related molecular workflows.
