The Amersham CyDye DIGE Cy5 Minimal Dye, 25 nmol (RPK0275) is a fluorescent protein labelling reagent for 2-D Fluorescence Difference Gel Electrophoresis. It is supplied as a 25 nmol Cy5 minimal dye pack, with the feed specifying a 5 × 5 nmol configuration for DIGE protein abundance comparison workflows.
Key Features
- Cy5 minimal dye for 2-D DIGE, designed for fluorescent labelling of proteins before two-dimensional electrophoresis
- 25 nmol total dye quantity, supplied as a 5 × 5 nmol pack configuration for RPK0275
- Size- and charge-matched DIGE fluor design, supporting co-migration of labelled protein samples within the same gel
- NHS ester labelling chemistry, designed to attach to the epsilon amino group of lysine residues by amide linkage
- Minimal labelling approach, with approximately 3% of total protein labelled under the described DIGE method
- Red Cy5 fluorescence channel, listed with 645 nm maximum absorption and 664 nm maximum fluorescence wavelength in the CyDye DIGE data file
- Protected frozen storage, specified for storage protected from light at -15 °C to -30 °C
Note: Anhydrous dimethylformamide (DMF) is required for use and should be ordered separately.
Benefits
- Enables comparison of protein abundance differences using multiplexed 2-D DIGE workflows.
- Helps reduce gel-to-gel variation by allowing differently labelled samples to run under the same electrophoresis conditions.
- Supports accurate within-gel matching by using matched fluor chemistry across DIGE minimal dyes.
- Provides a practical 25 nmol quantity for laboratories running repeated Cy5 minimal dye labelling work.
Why Choose the Amersham CyDye DIGE Cy5 Minimal Dye, 25 nmol (RPK0275)?
The Amersham CyDye DIGE Cy5 Minimal Dye, 25 nmol (RPK0275) is suited to laboratories using Cy5 minimal dye in 2-D DIGE protein analysis. John Morris Group can help confirm availability, handling requirements and suitability for Australian electrophoresis and protein analysis workflows.
