The Amersham CyDye DIGE Fluor Cy3 Minimal Dye, 25 nmol (RPK0273) is a fluorescent protein labelling reagent for 2-D Fluorescence Difference Gel Electrophoresis. This Cy3 minimal dye pack contains 25 nmol supplied as 5 x 5 nmol and is used for DIGE workflows where labelled protein samples and standards are analysed on the same 2-D electrophoresis gel.
Key Features
- Cy3 minimal dye for 2-D DIGE, designed for fluorescent protein labelling before two-dimensional electrophoresis separation
- 25 nmol total quantity, supplied as 5 x 5 nmol of CyDye DIGE Fluor Cy3 minimal dye
- Size- and charge-matched dye design, supporting co-migration of labelled samples within the same gel
- NHS ester reactive group, designed to covalently attach to the epsilon amino group of lysine residues through an amide linkage
- Minimal labelling approach, labelling approximately 1 to 2% of available lysine residues and typically one lysine per protein molecule
- Recommended 50 µg protein reaction, using 400 pmol of CyDye per 50 µg of protein in the standard protocol
- Suitable for larger experimental set-ups, with the pack specified as sufficient for up to 60 labelings
- Cold, dark storage, with storage specified at -15 °C to -30 °C and protection from light
Benefits
- Helps compare multiple protein samples and standards on the same 2-D electrophoresis gel.
- Supports reduced gel-to-gel variation by enabling multiplex DIGE experimental designs.
- Provides bright, sensitive fluorescent labelling with minimal signal loss during labelling, separation and scanning.
- Supports accurate spot matching by allowing each labelled protein sample to be visualised independently.
- Pack format suits larger studies with several variables or repeated labelling requirements.
Why Choose the Amersham CyDye DIGE Fluor Cy3 Minimal Dye, 25 nmol (RPK0273)?
The Amersham CyDye DIGE Fluor Cy3 Minimal Dye, 25 nmol (RPK0273) is a verified Cy3 DIGE reagent for protein labelling in 2-D electrophoresis workflows. John Morris Group supplies Amersham electrophoresis reagents across Australia with support for product selection and ordering.
