The Amersham CyDye DIGE Minimal Labeling Kit, 5 nmol (25801065) is a fluorescent protein labelling kit for 2-D Fluorescence Difference Gel Electrophoresis. It contains 5 nmol each of CyDye DIGE Fluor Cy2, Cy3 and Cy5 minimal dyes, which are size and charge matched for detecting protein abundance differences using Ettan DIGE workflows.
Key Features
- 5 nmol minimal labelling kit format, matching the verified Amersham CyDye DIGE Fluor kit variant for part 25801065
- Includes Cy2, Cy3 and Cy5 minimal dyes, with 5 nmol of each dye supplied in the kit
- Designed for 2-D DIGE protein analysis, supporting detection of protein abundance differences in 2-D Fluorescence Difference Gel Electrophoresis
- Size and charge matched fluorescent dyes, enabling co-migration of labelled protein samples within the gel
- Up to three prelabeled samples on one gel, supporting multiplexed comparison of protein samples and standards
- 50 µg protein labelling per reaction, based on the listed minimal dye labelling guidance
- Minimal cross-talk between fluors, helping support accurate multicolour analysis
- Storage at -15 °C to -30 °C, matching the verified content and storage specification
Benefits
- Helps compare protein abundance differences by labelling multiple samples for analysis on the same 2-D gel.
- Supports accurate within-gel matching because labelled samples experience the same first- and second-dimension running conditions.
- Bright, highly sensitive dyes support minimal labelling while limiting signal loss during labelling, separation and scanning.
- Discrete Cy2, Cy3 and Cy5 signals help reduce cross-talk in multicolour DIGE workflows.
- Suitable for general 2-D applications where sufficient sample amounts are available.
Why Choose the Amersham CyDye DIGE Minimal Labeling Kit, 5 nmol (25801065)?
The Amersham CyDye DIGE Minimal Labeling Kit, 5 nmol (25801065) is a verified Cytiva reagent kit for fluorescent protein labelling in 2-D DIGE analysis. John Morris Group supplies Amersham protein analysis reagents and electrophoresis consumables across Australia to support reliable comparative proteomics workflows.
