The IPG Buffer pH 3 to 11 NL, 1 mL (17600440), is an ampholyte-containing buffer concentrate for isoelectric focusing with Immobiline DryStrip gels. It is supplied as a 1 mL liquid aliquot and is used in rehydration solution to support first-dimension protein separation across the nonlinear pH 3 to 11 range.
Key Features
- pH 3 to 11 nonlinear buffer range, matched to broad-range Immobiline DryStrip IEF workflows
- 1 mL aliquot format, supplied as a liquid buffer concentrate for reagent preparation
- Ampholyte-containing formulation, specifically designed for use with Immobiline DryStrip gels
- Designed for IEF separation, helping maintain suitable conditions during first-dimension electrophoresis
- More uniform strip conductivity, supporting wider latitude in focusing run times
- Background staining reduction, helping improve clarity in downstream protein analysis workflows
- Flexible dilution use, diluted 50-fold or 200-fold in rehydration solution depending on the first-dimension system and strip pH range
- Cold storage format, specified for storage at 2 °C to 8 °C
Benefits
- Supports improved protein solubility when used with matching Immobiline DryStrip gels.
- Helps improve resolution and increase the probability of detecting low-abundance proteins.
- Provides a ready aliquot size for preparing rehydration solutions in 2-D electrophoresis workflows.
- Supports consistent first-dimension focusing conditions across a broad nonlinear pH range.
- Can help reduce high background staining in compatible protein analysis workflows.
Why Choose the IPG Buffer pH 3 to 11 NL, 1 mL (17600440)?
Choose the IPG Buffer pH 3 to 11 NL, 1 mL (17600440), when you need a nonlinear broad-range IPG buffer concentrate for Immobiline DryStrip isoelectric focusing and first-dimension protein analysis. John Morris Group can supply this IPG electrophoresis reagent for Australian laboratories working with 2-D electrophoresis and protein separation workflows.
