The DeStreak Reagent, 1 mL (17600318), is a reagent for use in 2-D electrophoresis workflows with Immobiline DryStrip gels. It helps prevent unspecific oxidation of protein thiol groups, reducing horizontal streaking and extra spots that can affect protein map quality, especially in higher pH regions.
Key Features
- 1 mL reagent format, supplied as DeStreak Reagent for electrophoresis workflow preparation
- Designed for 2-D electrophoresis, supporting improved protein map quality during Immobiline DryStrip runs
- Reduces unspecific oxidation, transforming protein thiol groups into stable disulphide groups
- Helps reduce streaking, particularly between spots in the pH 7 to 9 area
- Simplifies spot patterns, reducing extra spots caused by oxidation of proteins
- Compatible with most rehydration solutions, when reducing agents such as DTT or mercaptoethanol are removed
- Defined rehydration guidance, with 15 mg, equal to 12 µL, used per mL of rehydration solution without reducing agents
- Cold storage requirement, with storage specified at 4°C to 8°C
Benefits
- Helps improve reproducibility and quality of 2-D gel electrophoresis results.
- Supports clearer, better-resolved protein patterns by reducing oxidation-related artefacts.
- Provides a practical additive for rehydration solution preparation in compatible Immobiline DryStrip workflows.
- Can be used in analytical and preparative 2-D electrophoresis methods where streaking control is important.
Why Choose the DeStreak Reagent, 1 mL (17600318)?
The DeStreak Reagent, 1 mL (17600318), is suited to laboratories working with protein separation by isoelectric focusing and 2-D electrophoresis. John Morris Group supplies Cytiva protein analysis reagents across Australia, helping customers select suitable reagents for electrophoresis and Immobiline DryStrip workflows.
