The Amersham Anti-rabbit IgG Biotinylated Whole Antibody, 2 mL (RPN1004-2ML) is a species-specific biotinylated secondary antibody prepared from donkey anti-rabbit IgG. It is designed for immunodetection workflows including ELISA, immunocytochemistry and protein blotting, with application-specific dilution guidance supplied for relevant uses.
Key Features
- Biotinylated anti-rabbit IgG whole antibody, prepared from donkey antibody for use as a species-specific secondary reagent
- 2 mL pack format, supplied as the RPN1004-2ML variant for blotting and detection workflows
- Species-specific purification, affinity adsorbed to remove cross-reacting antibodies towards rat, human and mouse immunoglobulins
- Biotin attached via a spacer arm, with excess labelling reagent removed by extensive dialysis
- Optimised biotinylation level, selected to support streptavidin binding while retaining antigen binding ability
- Batch quality control, with cross-reactivity and antibody titre determined in an ELISA system
- Application-specific dilution guidance, including ELISA, immunocytochemistry and protein blotting examples
- Refrigerated storage, specified at 2 to 8 °C with repeated freeze-thaw cycles avoided
Benefits
- Supports flexible biotin-streptavidin detection using enzyme, fluorochrome, bridge or streptavidin-biotinylated enzyme complex systems.
- Helps reduce unwanted cross-reactivity in workflows where immunoglobulins from other species may be present.
- Provides a practical secondary antibody option for ELISA, immunocytochemistry and antigen identification in protein blotting.
- Supplied with dilution guidance to help users optimise sensitivity for different assay formats.
- Quality-controlled batch performance supports more reproducible immunodetection results.
Why Choose the Amersham Anti-rabbit IgG Biotinylated Whole Antibody, 2 mL (RPN1004-2ML)?
The Amersham Anti-rabbit IgG Biotinylated Whole Antibody, 2 mL (RPN1004-2ML) is a verified secondary detection reagent for rabbit IgG workflows using biotin-streptavidin detection systems. John Morris Group supplies Amersham protein analysis and blotting reagents across Australia with support for product selection and ordering.
