The Amersham ECL Plex Goat Anti-Rabbit IgG-Cy3, 150 µg (28901106), is a goat-derived secondary antibody conjugated with the Cy3 fluorophore for fluorescent Western blotting. It is supplied in a 150 µg pack for use with up to 1000 cm² of membrane area and supports rabbit IgG detection in direct fluorescence workflows.
Key Features:
- Goat anti-rabbit IgG secondary antibody, designed for detection of rabbit primary antibodies in Western blotting workflows
- Cy3 fluorophore conjugate, with excitation and emission maxima of 550 nm and 570 nm respectively
- Direct fluorescent detection, avoiding the need for ancillary detection reagents during blot analysis
- 150 µg supply format, suitable for at least 1000 cm² of membrane area
- Defined antibody dilution range, with a recommended working range of 1:1250 to 1:4000 and a 1:2500 starting point
- Multiplexing capability, supporting dual-protein experiments when used with appropriate rabbit and mouse primary antibodies together with matched Cy3 and Cy5 secondary antibodies
Benefits
- Supports direct fluorescence-based Western blot detection without additional detection reagent steps.
- Helps simplify quantitative blotting workflows through a defined fluorescent secondary antibody format.
- Allows rabbit IgG targets to be detected alongside suitable mouse IgG targets in multiplex experiments.
- Provides sufficient antibody quantity for multiple fluorescent blotting workflows across a substantial membrane area.
Why Choose the Amersham ECL Plex Goat Anti-Rabbit IgG-Cy3, 150 µg (28901106)?
The Amersham ECL Plex Goat Anti-Rabbit IgG-Cy3, 150 µg (28901106), provides a Cy3-labelled secondary antibody format for fluorescent detection of rabbit primary antibodies. Its defined membrane coverage and recommended dilution range support consistent fluorescent Western blotting workflows. John Morris Group can assist with product selection and ordering for your blotting and detection requirements.
