The Whatman Protran BA83 Membrane, 0.2 µm, 82 mm, 50 Pack (10401316) is a pure nitrocellulose membrane circle for flow-through and blotting applications. This BA83 membrane has a 0.2 µm pore size, 82 mm diameter, and pack size of 50 for protein transfer, blotting, and molecular biology workflows requiring high binding and low background.
Key Features
- Defined 0.2 µm pore size, supporting retention of small proteins in blotting and transfer workflows
- 82 mm circle format, supplied as round Protran BA83 membrane discs for compatible blotting and screening setups
- Pure nitrocellulose construction, manufactured from 100% pure nitrocellulose for high protein binding capacity
- Pack of 50 membranes, suitable for repeated laboratory blotting and flow-through applications
- High binding capacity, with a verified binding capacity of 75 to 110 µg/cm2
- Low background performance, supporting strong signal-to-noise ratios without stringent washing conditions
- Triton-free matrix, supporting use in sensitive protein transfer and immunodetection workflows
- Broad detection compatibility, suitable for isotopic, chemiluminescent, colorimetric, and fluorescent detection methods
Benefits
- Helps improve sensitivity when working with small proteins by using the BA83 0.2 µm pore size.
- Supports clear blotting results through high protein binding and low background membrane performance.
- Simplifies preparation compared with PVDF membranes because Protran nitrocellulose does not require methanol pre-wetting.
- Provides a practical 82 mm circular format for screening many samples on one membrane.
- Supports molecular biology workflows including Western blotting, Southern blotting, Northern blotting, colony hybridisation, and plaque lift applications.
Why Choose the Whatman Protran BA83 Membrane, 0.2 µm, 82 mm, 50 Pack (10401316)?
This Whatman Protran BA83 membrane provides a verified 0.2 µm pure nitrocellulose option for blotting workflows requiring an 82 mm circular membrane. John Morris Group supplies Whatman molecular biology and filtration consumables to support reliable protein transfer, immunodetection, and membrane-based analysis across Australia.
