The Amersham ECL Prime Western Blotting Detection Reagent, 1000 cm² (RPN2232) is a chemiluminescent ECL reagent system for sensitive quantitative or confirmatory Western blot detection. Supplied for 1000 cm² of membrane, the kit includes 50 mL each of Solution A and Solution B for high sensitivity protein detection workflows.
Key Features
- High sensitivity Western blot detection, with a listed sensitivity of approximately 1 pg
- Stable chemiluminescent signal, supporting multiple exposures and larger experimental series
- Reduced antibody consumption, allowing highly diluted primary and secondary antibodies without reducing sensitivity
- 1000 cm² membrane coverage, suitable for routine Western blot detection work
- Two-solution reagent format, supplied as 50 mL each of Solution A and Solution B
- Defined reagent components, including Solution A as the luminol solution and Solution B as the peroxide solution
- Verified Western blot workflow compatibility, including guidance for Amersham Hybond P PVDF membranes, Hybond-ECL nitrocellulose membranes, Hyperfilm, Rainbow molecular weight markers and Amersham ECL DualVue Western blotting markers
Benefits
- Helps detect low abundance proteins in sensitive Western blot applications.
- Supports repeat imaging and longer handling time between detection and final exposure.
- Helps reduce reagent and antibody use in routine blotting workflows.
- Provides a practical 1000 cm² pack size for laboratories running regular membrane-based protein detection.
Why Choose the Amersham ECL Prime Western Blotting Detection Reagent, 1000 cm² (RPN2232)?
Amersham ECL Prime RPN2232 is a high sensitivity ECL Western blotting reagent designed for stable signal output, quantitative detection and efficient antibody use. John Morris Group supplies Amersham blotting standards and reagents to Australian laboratories requiring reliable protein analysis consumables.
