Amersham ECL Western Blotting Reagent, 6000 cm² Kit (RPN2134) is an enhanced chemiluminescence detection reagent set for routine Western blotting. The kit includes paired ECL Detection Reagents 1 and 2, with enough volume for 6000 cm² of membrane coverage. It is designed for reliable detection of medium and high expression proteins using chemiluminescence detection.
Key Features
- Enhanced luminol-based chemiluminescence detection, suitable for routine Western blotting workflows
- Large 6000 cm² membrane coverage, with 375 mL each of Detection Reagent 1 and Detection Reagent 2
- Convenient paired reagent format, supplied as 3 x 125 mL of ECL Detection Reagent 1 and 3 x 125 mL of ECL Detection Reagent 2
- High sensitivity detection, with approximately 10 pg sensitivity in a model system
- Higher sensitivity than colourimetric assays, with at least 10-fold improvement stated for this reagent family
- Flexible detection options, detectable on Hyperfilm ECL or with a CCD imager
- Colourless reagent presentation, with both Detection Reagent 1 and Detection Reagent 2 supplied as colourless liquids
- Cold storage requirement, stored at 2 to 8°C
Benefits
- Supports routine Western blot detection where a reliable ECL chemiluminescent signal is required.
- Helps laboratories detect medium and high expression proteins with greater sensitivity than colourimetric methods.
- Provides a larger reagent format for workflows requiring up to 6000 cm² total membrane coverage.
- Works with film-based and CCD imaging detection setups, supporting flexibility in existing laboratory workflows.
Why Choose the Amersham ECL Western Blotting Reagent, 6000 cm² Kit (RPN2134)?
The Amersham ECL Western Blotting Reagent, 6000 cm² Kit (RPN2134) combines established enhanced chemiluminescence chemistry with a practical reagent volume for routine Western blotting. John Morris Group supplies Cytiva and Amersham protein analysis reagents across Australia and can help confirm suitability and availability for your laboratory workflow.
