The Amersham ECL Western Blotting Kit, 1000 cm² (RPN2108) is a complete enhanced chemiluminescent detection system for Western blotting. The kit includes ECL detection reagents, HRP-conjugated anti-mouse and anti-rabbit antibodies, and blocking agent sufficient for detecting 1000 cm² of membrane.
Key Features
- Complete ECL detection kit, combining detection reagents, HRP secondary antibodies and blocking agent in one system
- 1000 cm² membrane coverage, equivalent to 10 blots measuring 10 cm x 10 cm
- Mouse and rabbit primary antibody detection, with HRP-linked anti-mouse and anti-rabbit whole antibodies supplied
- Luminol-based chemiluminescent detection, producing a signal after addition of ECL detection reagents
- Film and CCD detection compatible, allowing chemiluminescent signal capture using X-ray film or a CCD camera
- Nitrocellulose and PVDF membrane compatibility, supporting common Western blot membrane formats
- High sensitivity detection, with up to 10 times greater sensitivity than colorimetric methods
- Refrigerated storage, with all components stored at 2 °C to 8 °C on receipt
Benefits
- Simplifies Western blot detection by supplying the main ECL reagents and HRP secondary antibodies together.
- Supports detection of blots probed with either mouse or rabbit primary antibodies.
- Helps produce rapid chemiluminescent results suitable for film or digital imaging workflows.
- Reduces the need to source separate detection reagents, secondary antibodies and blocking agent for standard ECL workflows.
- Provides enough reagent capacity for routine detection across 1000 cm² of membrane area.
Why Choose the Amersham ECL Western Blotting Kit, 1000 cm² (RPN2108)?
The Amersham ECL Western Blotting Kit, 1000 cm² (RPN2108) is a verified Cytiva chemiluminescent Western blotting system supplied with detection reagents, HRP-labelled secondary antibodies and blocking agent. John Morris Group supplies Amersham and Cytiva blotting reagents for Australian laboratories requiring dependable protein detection consumables.
