The Amersham ECL Western Blotting Reagent, 4000 cm² (RPN2106) is an enhanced chemiluminescence detection reagent kit for Western blotting applications. It is supplied with 250 mL each of Detection Reagent 1 and Detection Reagent 2, sufficient for detection across 4000 cm² of membrane.
Key Features
- Enhanced luminol-based ECL detection, suitable for routine Western blotting workflows
- 4000 cm² membrane coverage, supplied for larger detection workloads or repeated blotting runs
- Two-reagent kit format, with 250 mL each of Detection Reagent 1 and Detection Reagent 2
- High sensitivity detection, listed at approximately 10 pg in a model system
- Up to 2 hour emission duration, supporting film or CCD image capture after substrate reaction
- Compatible detection options, detectable on Hyperfilm ECL or with a CCD imager
- Recommended with Hybond ECL membrane, supporting a matched Western blotting detection workflow
- Cold storage requirement, specified for storage at 2 to 8 °C
Note: The product documentation states this reagent is intended for research use and is not for clinical or in vitro diagnostic procedures.
Benefits
- Supports reliable chemiluminescent detection of medium and high expression proteins.
- Helps laboratories move beyond colourimetric detection with higher sensitivity for Western blot workflows.
- Provides a practical 4000 cm² pack size for facilities running multiple membrane detection cycles.
- Works with common film and CCD imaging approaches for flexible detection setup planning.
- Pairs with Hybond ECL membrane for laboratories standardising Cytiva Western blotting reagents and consumables.
Why Choose the Amersham ECL Western Blotting Reagent, 4000 cm² (RPN2106)?
The Amersham ECL Western Blotting Reagent, 4000 cm² (RPN2106) is suited to laboratories requiring enhanced chemiluminescence detection for routine Western blotting. John Morris Group can help confirm availability, handling requirements and suitability for Australian protein analysis and blotting workflows.
