pI Markers High pI Kit pH 5 to 10.5, 10 Vials (17047301) is a lyophilised protein marker kit for isoelectric focusing calibration. It is supplied as 10 vials, with 280 µg per vial, for pI determination across the high pI range.
Key Features
- High pI range from pH 5 to 10.5, suited to IEF calibration in the upper pI interval
- Defined pI marker span, covering pI 5.2 to 10.25 at 24°C
- Lyophilised protein marker format, supplied as stable, salt-free, highly purified proteins
- 10 vial kit format, with 280 µg of marker material per vial
- Supports isoelectric focusing calibration, allowing marker lanes to be run alongside experimental samples
- Visual focusing monitoring, supported by coloured markers including myoglobin bands and Cytochrome C
- Reconstitution guidance provided, using 100 µL of distilled or deionised water, or 50 µL for PhastGel use
Note: This kit is not recommended for denaturing systems such as urea-based methods.
Benefits
- Helps calibrate pH gradients during IEF so protein pI values can be determined more confidently.
- Provides a focused high-range kit when the expected pI values sit above the mid-range region.
- Supports clearer interpretation by placing reference markers in parallel with unknown samples.
- Reduces setup uncertainty with defined marker pI values and practical reconstitution instructions.
Why Choose the pI Markers High pI Kit pH 5 to 10.5, 10 Vials (17047301)?
Choose pI Markers High pI Kit pH 5 to 10.5, 10 Vials (17047301) when you need a defined high-range marker kit for IEF pI calibration. John Morris Group supplies Cytiva pI marker reagents for Australian laboratories working in protein analysis and electrophoresis workflows.
