HiScreen Capto Butyl ImpRes Column, 4.7 mL (17371910) is a ready-to-use prepacked chromatography column for hydrophobic interaction chromatography method development. The 4.7 mL HiScreen format is prepacked with Capto Butyl ImpRes resin and supports parameter screening, method optimisation, and scale-up studies using the same linear flow velocity.
Key Features
- Ready-to-use 4.7 mL HiScreen format, supplied as one prepacked column for process development work
- Capto Butyl ImpRes HIC resin, designed for intermediate and polishing steps in downstream protein purification
- High-flow agarose matrix, combining pressure-flow performance with high-resolution separation capability
- Butyl hydrophobic ligand, providing HIC selectivity for biomolecules with exposed hydrophobic groups
- 40 µm average particle size, supporting higher-resolution polishing compared with larger-particle HIC resins
- 10 cm bed height, suited to method optimisation, parameter screening, and linear scale-up studies
- 37 mg BSA/mL resin binding capacity, measured at 10% breakthrough under stated Cytiva test conditions
- Broad pH stability, with pH 3 to 13 operational stability and pH 2 to 14 short-term CIP stability
Benefits
- Helps screen HIC conditions while reducing sample and buffer consumption compared with larger column formats.
- Supports fast method development before moving to larger prepacked or packed process columns.
- Allows two HiScreen columns to be connected in series when a 20 cm bed height is required.
- Provides reproducible, scalable results when transferred to BioProcess columns packed with the same resin.
- Supports purification workflows involving proteins, peptides, and other hydrophobic biomolecules.
Why Choose the HiScreen Capto Butyl ImpRes Column, 4.7 mL (17371910)?
The HiScreen Capto Butyl ImpRes Column, 4.7 mL (17371910) is suited to laboratories developing hydrophobic interaction chromatography methods for downstream protein purification. John Morris Group supplies Cytiva chromatography consumables to Australian laboratories with support for product selection and ordering.
