The HiScreen MabSelect Column, 1 x 4.7 mL (28926973) is a prepacked affinity chromatography column for capture of monoclonal antibodies during process development. The column is packed with MabSelect protein A resin in a 4.7 mL HiScreen format for method optimisation, parameter screening, and scale-up studies.
Key Features
- Prepacked HiScreen column format, supplied as 1 x 4.7 mL for convenient process development workflows
- MabSelect protein A affinity resin, designed for fast purification of monoclonal antibodies from large sample volumes
- 4.7 mL column volume, with 0.77 x 10 cm column dimensions for scalable experiments at relevant process flow rates
- Recombinant protein A ligand, produced in E. coli and coupled by epoxy activation
- Highly cross-linked agarose matrix, with spherical particles and a median particle size of 85 µm
- Defined binding capacity, approximately 30 mg human IgG/mL resin under stated test conditions
- Process development flow range, with recommended operating flow velocity of 75 to 300 cm/h and maximum operating flow velocity of 500 cm/h
- Biocompatible polypropylene column body, with a column hardware pressure limit of 8 bar, 0.8 MPa, 117 psi
Benefits
- Supports method optimisation and parameter screening for monoclonal antibody capture workflows.
- Helps reduce sample and buffer consumption while maintaining a process-relevant 10 cm bed height.
- Can be connected in series with a union when a 20 cm bed height is required for further method work.
- Provides a ready-to-use format for evaluating MabSelect resin before scale-up to larger chromatography formats.
Why Choose the HiScreen MabSelect Column, 1 x 4.7 mL (28926973)?
The HiScreen MabSelect Column, 1 x 4.7 mL (28926973) is suited to laboratories developing and optimising protein A affinity capture methods for monoclonal antibodies. John Morris Group supplies Cytiva chromatography columns to support antibody purification and process development workflows across Australian laboratories.
