The MabSelect PrismA X HiTrap Column, 1 × 5 mL (17550153), is a prepacked HiTrap column containing MabSelect PrismA X protein A resin for monoclonal antibody capture. The resin combines a highly cross-linked agarose matrix with an alkali-stable protein A-derived ligand to support high binding capacity and repeated cleaning-in-place. The single 5 mL column format is suited to small-scale evaluation, process development, and rapid cycling chromatography studies before scale-up.
Key Features
- Prepacked 5 mL HiTrap format, supplied as one ready-to-use column for small-scale protein A affinity chromatography
- MabSelect PrismA X resin, designed for efficient capture of monoclonal antibodies
- Enhanced dynamic binding capacity, with more than 80 mg mAb/mL resin at 6 min residence time and more than 74 mg mAb/mL resin at 4 min residence time
- Alkali-stable protein A-derived ligand, supporting cleaning-in-place with 0.5 to 1.0 M NaOH
- Highly cross-linked agarose matrix, supplied as spherical resin beads for affinity chromatography workflows
- Smaller 50 µm median particle size, helping enable fast mAb uptake and high capacity at short residence times
- Short bed height suitability, designed for rapid cycling chromatography applications to improve protein A capture productivity
- Broad pH stability, with operational stability from pH 3 to 12 and CIP stability from pH 2 to 14
Benefits
- Supports fast evaluation of MabSelect PrismA X performance in a convenient prepacked column format.
- Helps reduce manual packing work during early protein A capture method development.
- Allows laboratories to assess short residence time and rapid cycling strategies before moving to larger formats.
- Supports robust cleaning and sanitisation workflows where repeated use is required.
- Provides a practical development path alongside bulk MabSelect PrismA X resin, HiScreen columns, and larger production formats.
Why Choose the MabSelect PrismA X HiTrap Column, 1 × 5 mL (17550153)?
The MabSelect PrismA X HiTrap Column, 1 × 5 mL (17550153), is a strong option for laboratories developing high-capacity and robust protein A capture workflows in a ready-to-use HiTrap format. John Morris Group supplies Cytiva chromatography columns and resins to Australian laboratories with support for product selection and ordering.
