The HiScreen MabSelect PrismA X Column, 1 x 4.7 mL (17550115) is a prepacked protein A affinity chromatography column for monoclonal antibody capture and process development. It is packed with MabSelect PrismA X resin, a high-capacity protein A resin with excellent alkaline stability for repeated cleaning in place and robust mAb purification workflows.
Key Features
- Prepacked 4.7 mL HiScreen format, supplied as one 4.7 mL column for method optimisation and parameter screening
- MabSelect PrismA X protein A resin, designed for high-capacity capture of monoclonal antibodies in protein A chromatography
- Short bed height application support, suited to rapid cycling chromatography workflows for improved protein A capture productivity
- High dynamic binding capacity, with QB10% greater than 80 mg mAb/mL resin at 6 min residence time and greater than 74 mg mAb/mL resin at 4 min
- Defined HiScreen column geometry, with 7.7 x 100 mm bed dimensions, 100 mm bed height, and 7.7 mm inner diameter
- Highly cross-linked agarose matrix, with a spherical base matrix and approximately 50 µm median particle size
- Alkali-stable protein A ligand, using a protein A-derived E. coli ligand with single-point attachment and epoxy coupling chemistry
- Wide pH and CIP stability, with operational pH stability from 3 to 12 and CIP stability from pH 2 to 14
Benefits
- Supports efficient mAb capture screening with a ready-to-use prepacked HiScreen column format.
- Helps reduce resin and buffer consumption through high binding capacity at practical residence times.
- Enables development work for rapid cycling chromatography and short bed height applications.
- Allows robust alkaline cleaning with 0.5 to 1.0 M NaOH to help reduce bioburden between runs.
- Provides a scalable process development format using the same MabSelect PrismA X resin chemistry available in larger formats.
Why Choose the HiScreen MabSelect PrismA X Column, 1 x 4.7 mL (17550115)?
The HiScreen MabSelect PrismA X Column, 1 x 4.7 mL (17550115) is suited to laboratories developing mAb capture processes where high binding capacity, alkaline cleaning, and scale-up relevance are important. John Morris Group supplies Cytiva chromatography columns and resins to Australian laboratories and can assist with product selection, ordering, and availability.
