The MabSelect PrismA X Protein A Resin, 25 mL (17550101), is a high-capacity protein A affinity chromatography resin for monoclonal antibody capture. It uses a highly cross-linked agarose matrix with an alkali-stable protein A-derived ligand to support strong binding capacity and repeated cleaning-in-place. The 25 mL pack is suited to process development, small-scale evaluation, and method optimisation before scale-up.
Key Features
- High-capacity protein A resin, designed for efficient capture of monoclonal antibodies
- Enhanced dynamic binding capacity, with more than 80 mg mAb/mL resin at 6 min residence time and more than 74 mg mAb/mL resin at 4 min residence time
- Alkali-stable protein A-derived ligand, supporting repeated cleaning-in-place using 0.5 to 1.0 M NaOH
- Highly cross-linked agarose matrix, supplied as spherical resin beads for affinity chromatography workflows
- Smaller 50 µm median particle size, helping enable fast mAb uptake and high capacity at short residence times
- Short bed height suitability, designed for rapid cycling chromatography applications to improve capture-step productivity
- Flexible flow performance, with recommended maximum operating flow velocity of 220 cm/h at 20 cm bed height and 400 cm/h at 10 cm bed height
- Broad pH stability, with operational stability from pH 3 to 12 and CIP stability from pH 2 to 14
Benefits
- Supports high mass throughput per resin volume during monoclonal antibody capture process development.
- Helps reduce resin and buffer consumption when high dynamic binding capacity is required.
- Enables robust cleaning and sanitisation strategies for repeated protein A chromatography cycles.
- Provides a useful development format for evaluating short bed height and rapid cycling chromatography strategies.
- Supports scale-up planning across MabSelect PrismA X resin and prepacked column formats.
Why Choose the MabSelect PrismA X Protein A Resin, 25 mL (17550101)?
The MabSelect PrismA X Protein A Resin, 25 mL (17550101), is a strong option for laboratories developing high-capacity and robust protein A capture workflows for mAb purification. John Morris Group supplies Cytiva chromatography resins to Australian laboratories with support for product selection and ordering.
