The HiTrap MabSelect PrismA Column, 5 x 5 mL (17549854) is a ready-to-use prepacked protein A affinity chromatography column for monoclonal antibody and Fc-containing recombinant protein purification. It is packed with MabSelect PrismA resin, an alkali-tolerant protein A resin based on a rigid, highly cross-linked agarose matrix for high binding capacity and efficient cleaning between runs.
Key Features
- Prepacked 5 x 5 mL HiTrap format, supplied as five 5 mL columns for preparative antibody purification workflows
- MabSelect PrismA protein A resin, designed for monoclonal antibody purification with enhanced alkali and protease stability
- High dynamic binding capacity, with approximately 40 mg polyclonal IgG/mL resin at 2 min residence time and approximately 80 mg polyclonal IgG/mL resin at 6 min residence time
- Rigid agarose bead matrix, using highly cross-linked agarose with approximately 60 µm median particle size
- 5 mL HiTrap column geometry, with 1.6 x 2.5 cm column dimensions and a 0.5 MPa pressure limit
- Defined 5 mL column flow rates, with recommended operating flow rate of 2.5 mL/min and maximum operating flow rate of 20 mL/min
- Wide pH stability range, with operational pH stability from 3 to 12 and CIP pH stability from 2 to 14
- Alkaline cleaning support, allowing cleaning-in-place with 0.5 to 1.0 M sodium hydroxide
Benefits
- Supports efficient one-step capture of monoclonal antibodies and Fc-containing recombinant proteins.
- Helps reduce preparation time with a ready-to-use prepacked column format.
- Supports repeated purification work where cleaning between runs is important.
- Provides high capacity for preparative antibody purification while maintaining convenient HiTrap handling.
- Can be operated using a syringe, peristaltic pump, or chromatography system for flexible lab workflows.
Why Choose the HiTrap MabSelect PrismA Column, 5 x 5 mL (17549854)?
The HiTrap MabSelect PrismA Column, 5 x 5 mL (17549854) is suited to laboratories purifying monoclonal antibodies, bispecific antibodies, and Fc-containing recombinant proteins where binding capacity and alkaline cleaning are important. John Morris Group supplies Cytiva prepacked chromatography columns to Australian laboratories and can assist with product selection, ordering, and availability.
