The HiTrap MabSelect PrismA Protein A Column, 1 x 5 mL (17549853), is a ready-to-use HiTrap column prepacked with MabSelect PrismA Protein A affinity chromatography resin. It is designed for capture of monoclonal antibodies and Fc-containing recombinant proteins, with enhanced alkaline stability to support efficient cleaning between purification runs.
Key Features
- Ready-to-use HiTrap format, prepacked with MabSelect PrismA resin for Protein A affinity chromatography workflows
- 1 x 5 mL column size, supplied as one 5 mL column for preparative purification and method development
- Biocompatible polypropylene hardware, designed for biomolecule chromatography without opening or refilling the column
- Flexible operation, suitable for use with a syringe, peristaltic pump, or chromatography system
- Engineered Protein A-derived ligand, produced in E. coli and designed for enhanced alkali and protease stability
- High dynamic binding capacity, with approximately 80 mg polyclonal IgG/mL resin at 6 minutes residence time
- Defined 5 mL flow performance, with a recommended operating flow rate of 2.5 mL/min and maximum operating flow rate of 20 mL/min
- Strong CIP compatibility, allowing use of 0.5 to 1.0 M sodium hydroxide for cleaning-in-place workflows
Benefits
- Reduces preparation time by supplying MabSelect PrismA resin in a convenient prepacked column format.
- Supports efficient antibody capture where binding capacity, alkaline stability, and cleaning between runs are important.
- Helps process development teams evaluate Protein A purification conditions before moving to larger formats.
- Provides a practical format for monoclonal antibody and many bispecific antibody purification workflows.
- Supports consistent small-scale chromatography work with defined column volume, pressure, and flow characteristics.
Why Choose the HiTrap MabSelect PrismA Protein A Column, 1 x 5 mL (17549853)?
The HiTrap MabSelect PrismA Protein A Column, 1 x 5 mL (17549853), is suited to laboratories developing antibody capture workflows using a high-capacity, alkali-tolerant Protein A resin. John Morris Group supplies Cytiva chromatography columns across Australia, helping customers select suitable prepacked columns for downstream purification and process development.
