The HiTrap MabSelect PrismA Column, 5 × 1 mL (17549852), is a set of prepacked affinity chromatography columns for monoclonal antibody and Fc-containing recombinant protein capture. Each 1 mL HiTrap column is prepacked with MabSelect PrismA, an alkali-tolerant protein A chromatography resin. The 5 × 1 mL format is suited to screening, method development, and preparative purification where efficient cleaning between runs is important.
Key Features
- Prepacked 1 mL HiTrap format, supplied as five ready-to-use columns for small-scale affinity chromatography
- MabSelect PrismA resin, designed for capture of monoclonal antibodies and Fc-containing recombinant proteins
- Biocompatible polypropylene hardware, with columns suitable for operation using a syringe, peristaltic pump, or chromatography system
- Defined 1 mL column geometry, with 0.7 × 2.5 cm column dimensions
- Alkali-tolerant protein A ligand, derived from E. coli and engineered for enhanced alkali and protease stability
- Rigid agarose matrix, using highly cross-linked agarose with a median particle size of approximately 60 µm
- Defined binding capacity, with approximately 40 mg polyclonal IgG/mL resin at 2 min residence time under Cytiva test conditions
- CIP-compatible pH stability, with operational stability from pH 3 to 12 and CIP stability from pH 2 to 14
Benefits
- Supports efficient small-scale evaluation of MabSelect PrismA before scale-up to larger column or resin formats.
- Reduces column packing work by providing a ready-to-use prepacked format.
- Helps develop robust antibody capture methods where cleaning between purification runs is important.
- Supports purification workflows for monoclonal antibodies and many bispecific antibody formats where protein A capture is suitable.
- Provides a convenient format for comparing binding, washing, elution, and CIP conditions during method optimisation.
Why Choose the HiTrap MabSelect PrismA Column, 5 × 1 mL (17549852)?
The HiTrap MabSelect PrismA Column, 5 × 1 mL (17549852), is a practical choice for laboratories developing protein A capture methods using MabSelect PrismA resin in a ready-to-use HiTrap format. John Morris Group supplies Cytiva chromatography columns to Australian laboratories with support for product selection and ordering.
