The HiTrap MabSelect PrismA Column, 1 x 1 mL (17549851), is a prepacked affinity chromatography column containing MabSelect PrismA protein A chromatography resin. It is designed for monoclonal antibody purification workflows where high binding capacity, alkaline stability, and efficient cleaning between purification runs are important.
Key Features
- Prepacked 1 x 1 mL HiTrap format, supplied as one 1 mL column for small-scale antibody purification and process development
- MabSelect PrismA protein A resin, using an alkali-tolerant protein A-derived ligand produced in E. coli
- Rigid, highly cross-linked agarose matrix, supporting robust affinity chromatography performance
- Approximately 60 µm particle size, based on the median particle size of the cumulative volume distribution
- Epoxy coupling chemistry, used for the MabSelect PrismA ligand on the resin matrix
- Approximately 40 mg polyclonal IgG/mL resin binding capacity, measured at 10% breakthrough with a 2 min residence time
- 1 mL column hardware format, with 0.7 x 2.5 cm column dimensions and a 0.5 MPa pressure limit
- Broad resin pH stability, with pH 3 to 12 operational stability and pH 2 to 14 CIP stability
Benefits
- Supports efficient small-scale purification of monoclonal antibodies using a ready-to-use protein A column format.
- Helps reduce column preparation time compared with manually packing affinity resin into small columns.
- Supports cleaning-in-place using 0.5 to 1.0 M sodium hydroxide for suitable workflows.
- Can be operated with a syringe, peristaltic pump, or chromatography system for flexible laboratory use.
Why Choose the HiTrap MabSelect PrismA Column, 1 x 1 mL (17549851)?
Choose the HiTrap MabSelect PrismA Column, 1 x 1 mL (17549851), when your workflow needs a compact prepacked protein A column for monoclonal antibody purification and method development. John Morris Group can support Australian laboratories with Cytiva chromatography product selection, ordering, and related workflow requirements.
