The MabSelect PrismA Protein A Resin, 200 mL (17549802), is a bulk Protein A affinity chromatography resin for antibody purification workflows. It uses an optimised high-flow agarose base matrix and genetically engineered Protein A-derived ligand to support high binding capacity, alkaline stability, and process economy in monoclonal antibody purification.
Key Features
- Bulk 200 mL resin format, supplied for packing into suitable chromatography columns
- Protein A affinity resin, designed for antibody capture in downstream purification workflows
- Optimised high-flow agarose matrix, supporting productive operation in packed-bed chromatography
- Engineered Protein A-derived ligand, alkaline stabilised and produced in E. coli
- Approximately 60 µm particle size, based on median particle size of the cumulative volume distribution
- High dynamic binding capacity, with approximately 80 mg human IgG/mL resin at 6 min residence time and approximately 65 mg human IgG/mL resin at 4 min residence time
- Defined operating flow performance, with a recommended maximum operating flow velocity of 300 cm/h
- Strong cleaning-in-place stability, with CIP pH stability from pH 2 to 14 and compatibility with 0.5 to 1.0 M sodium hydroxide cleaning approaches
Benefits
- Helps increase antibody mass throughput per resin volume in Protein A capture steps.
- Supports robust cleaning and sanitisation practices for improved process economy and bioburden control.
- Can help reduce required resin volume and buffer consumption for a given mass throughput.
- Provides a scalable resin platform for monoclonal antibody and many bispecific antibody purification workflows.
Why Choose the MabSelect PrismA Protein A Resin, 200 mL (17549802)?
The MabSelect PrismA Protein A Resin, 200 mL (17549802), is suited to laboratories and bioprocess teams developing high-capacity antibody capture steps. John Morris Group supplies Cytiva chromatography resins across Australia, helping customers select suitable affinity media for downstream purification and process development workflows.
