The MabSelect PrismA Protein A Resin, 25 mL (17549801), is an affinity chromatography resin for monoclonal antibody and Fc-containing protein capture. It uses a highly cross-linked agarose matrix with an alkaline stabilised protein A-derived ligand to support high binding capacity and robust cleaning workflows. The 25 mL pack is suitable for process development, small-scale evaluation and method optimisation before scale-up.
Key Features
- Protein A affinity resin, designed for capture of monoclonal antibodies and Fc-containing recombinant proteins
- High binding capacity, with approximately 80 mg human IgG/mL resin at 6 min residence time and approximately 65 mg human IgG/mL resin at 4 min residence time
- Alkaline stabilised ligand, using a protein A-derived E. coli ligand for robust antibody purification workflows
- Highly cross-linked agarose matrix, supplied as spherical resin beads for chromatography applications
- Defined particle size, with a median particle size of approximately 60 µm
- Recommended operating flow velocity, up to 300 cm/h under Cytiva test conditions
- Broad pH stability, with operational stability from pH 3 to 12 and CIP stability from pH 2 to 14
- Standard delivery condition, supplied in 20% ethanol
Benefits
- Supports high mass throughput per resin volume unit during antibody capture process development.
- Helps reduce process footprint and buffer use when high dynamic binding capacity is required.
- Enables robust cleaning and sanitisation strategies for repeated protein A chromatography cycles.
- Provides a scalable resin platform for moving from small-scale evaluation to larger mAb purification workflows.
- Supports purification of many monoclonal and bispecific antibody formats where protein A capture is suitable.
Why Choose the MabSelect PrismA Protein A Resin, 25 mL (17549801)?
The MabSelect PrismA Protein A Resin, 25 mL (17549801), is a strong option for laboratories developing high-capacity protein A capture methods for antibody purification. John Morris Group supplies Cytiva chromatography resins to Australian laboratories with support for product selection and ordering.
