The HiTrap MabSelect VH3 Column, 5 x 5 mL (17549354) is a prepacked protein A affinity chromatography column for efficient separation of bispecific antibodies, Fab, scFv, and VHH that contain the VH3 sequence family. It is packed with MabSelect VH3 resin, using an engineered protein A-derived ligand that interacts with the VH3 region while avoiding Fc-region interaction.
Key Features
- Prepacked 5 x 5 mL HiTrap format, supplied as five 5 mL columns for convenient affinity chromatography workflows
- VH3-specific engineered ligand, with protein A-derived interaction enhanced for the VH3 region and no interaction with the Fc region
- High binding capacity, with QB10% of approximately 70 mg mAb/mL resin at 6 min residence time and approximately 60 mg mAb/mL resin at 4 min residence time
- Rigid agarose bead format, using a highly cross-linked spherical agarose matrix with approximately 60 µm median particle size
- Good impurity resolution, supporting capture-stage separation of bispecific antibodies from product-related impurities
- Strong alkaline stability, stable for cleaning with 0.5 M NaOH and with CIP pH stability from 2.5 to 13.7
- Wide operating pH window, with operational pH stability from 3 to 12
- Delivered in storage solution, supplied in 20% ethanol
Benefits
- Supports efficient capture of antibody fragments and bispecific antibodies containing the VH3 sequence family.
- Helps separate target bispecific antibodies from unwanted mispaired antibodies and fragments.
- Provides a ready-to-use prepacked format that reduces preparation time compared with packing columns manually.
- Supports screening and small-scale purification work before transfer to larger MabSelect VH3 formats.
- Allows harsh alkaline cleaning conditions to help manage carryover and bioburden risk in repeated use workflows.
Why Choose the HiTrap MabSelect VH3 Column, 5 x 5 mL (17549354)?
The HiTrap MabSelect VH3 Column, 5 x 5 mL (17549354) is suited to laboratories working with VH3-containing bispecific antibodies, Fab, scFv, and VHH formats where selective affinity capture is important. John Morris Group supplies Cytiva prepacked chromatography columns to Australian laboratories and can assist with product selection, ordering, and availability.
