The HiTrap MabSelect VH3 Protein A Column, 1 x 5 mL (17549353), is a prepacked HiTrap column containing MabSelect VH3 affinity resin for chromatography workflows involving VH3 sequence family antibodies and fragments. Its engineered protein A-derived ligand is designed to avoid Fc-region interaction while enhancing VH3-region interaction for applications such as bispecific antibody and antibody-fragment purification.
Key Features
- Ready-to-use HiTrap column format, prepacked with MabSelect VH3 resin for affinity chromatography workflows
- 1 x 5 mL column size, supplied as one 5 mL column for method development and small-scale purification
- VH3-focused affinity interaction, designed for the variable heavy chain of the VH3 sequence family
- Engineered protein A-derived ligand, produced in E. coli with no Fc-region interaction and enhanced VH3-region interaction
- Highly cross-linked agarose matrix, supplied as spherical resin beads within the prepacked column
- Approximately 60 µm particle size, based on median particle size of the cumulative volume distribution
- High dynamic binding capacity, with approximately 70 mg mAb/mL resin at 6 min residence time and approximately 60 mg mAb/mL resin at 4 min residence time
- Strong alkaline stability, with operational pH stability from pH 3 to 12 and CIP pH stability from pH 2.5 to 13.7
Benefits
- Helps screen VH3-specific affinity purification conditions before moving to larger resin formats.
- Supports separation strategies for bispecific antibodies and antibody fragments such as Fab, scFv, and VHH.
- Reduces preparation time by providing MabSelect VH3 resin in a convenient prepacked column format.
- Supports development work where avoiding conventional Fc-region protein A interaction is important.
Why Choose the HiTrap MabSelect VH3 Protein A Column, 1 x 5 mL (17549353)?
The HiTrap MabSelect VH3 Protein A Column, 1 x 5 mL (17549353), is suited to laboratories developing purification workflows for VH3-containing bispecific antibodies and antibody fragments. John Morris Group supplies Cytiva chromatography columns across Australia, helping customers select suitable affinity media for method development and downstream purification workflows.
