The MabSelect VH3 Protein A Affinity Resin, 25 mL (17549301), is a bulk affinity chromatography resin for purification workflows involving VH3 sequence family antibody molecules and fragments. It uses an engineered protein A-derived ligand that has no interaction with the Fc region and enhanced interaction with the VH3 region.
Key Features
- Bulk 25 mL resin format, supplied for packing into suitable chromatography columns
- VH3-specific affinity interaction, designed to bind the variable heavy chain of the VH3 sequence family
- No Fc-region interaction, helping support separation strategies where conventional protein A binding is not desired
- Engineered protein A-derived ligand, produced in E. coli and coupled to the matrix by single-point attachment
- Highly cross-linked agarose matrix, supplied as spherical resin beads for packed-bed chromatography
- Approximately 60 µm particle size, based on median particle size of the cumulative volume distribution
- High dynamic binding capacity, with approximately 70 mg mAb/mL resin at 6 min residence time and approximately 60 mg mAb/mL resin at 4 min residence time
- Strong alkaline stability, with suitability for cleaning-in-place conditions including 0.5 M sodium hydroxide
Benefits
- Supports efficient purification of bispecific antibodies and antibody fragments containing VH3 sequence family domains.
- Helps separate target bispecific antibodies from product-related impurities such as unwanted mispaired antibodies and fragments.
- Provides a practical bulk resin format for screening, method development, and scale-up preparation.
- Offers defined resin characteristics for users developing affinity capture steps in downstream purification workflows.
Why Choose the MabSelect VH3 Protein A Affinity Resin, 25 mL (17549301)?
The MabSelect VH3 Protein A Affinity Resin, 25 mL (17549301), is suited to laboratories working with VH3-containing antibody fragments, Fab, scFv, VHH, and bispecific antibody purification strategies. John Morris Group supplies Cytiva chromatography resins across Australia, helping customers select suitable affinity media for process development and downstream purification workflows.
