The HiScreen Capto L Affinity Column, 1 x 4.7 mL (17547814) is a prepacked affinity chromatography column for capture of antibodies and antibody fragments. Packed with Capto L resin, it is designed for method optimisation, parameter screening, and small-scale purification of antibody formats such as Fab fragments, scFv, and domain antibodies.
Key Features
- Prepacked HiScreen format, supplied as a 1 x 4.7 mL column for convenient process development and small-scale purification
- Capto L affinity resin, designed for capture of antibodies and antibody fragments
- Kappa light chain binding specificity, using recombinant Protein L with affinity for the variable region of antibody kappa light chains
- High dynamic binding capacity, with approximately 25 mg Fab/mL medium at 10% breakthrough under stated test conditions
- Rigid high-flow agarose matrix, supporting productivity, process economy, and process design flexibility
- 85 µm median particle size, based on the d50v average particle size of the Capto L resin
- Epoxy coupling chemistry, used to attach the recombinant Protein L ligand to the matrix
- Process development column design, with 10 cm bed height and the option to connect columns in series to achieve 20 cm bed height
Benefits
- Supports efficient capture of a broad range of antibody fragments, including Fab fragments, scFv, and domain antibodies.
- Helps reduce development time by providing a ready-to-use column format for screening and optimisation.
- Minimises sample and buffer consumption through the small 4.7 mL bed volume.
- Supports scalable method development using the same resin chemistry as larger BioProcess column formats.
- Helps improve antibody fragment purity through high specificity and low ligand leakage characteristics.
Why Choose the HiScreen Capto L Affinity Column, 1 x 4.7 mL (17547814)?
The HiScreen Capto L Affinity Column, 1 x 4.7 mL (17547814) is suited to laboratories developing affinity capture methods for antibody fragments and related formats. John Morris Group supplies Cytiva HiScreen columns and chromatography resins for Australian laboratories and can help confirm suitability for your antibody purification workflow.
