The Capto L Antibody Fragment Resin, 200 mL (17547802) is a protein L affinity chromatography resin for capture of antibodies and antibody fragments containing a kappa light chain. It combines a rigid high-flow agarose matrix with a recombinant protein L ligand, supporting purification of formats such as Fabs, single-chain variable fragments and domain antibodies.
Key Features
- 200 mL bulk resin format, supplied for packing into suitable chromatography columns during antibody fragment purification development or process work
- Protein L affinity chemistry, using recombinant protein L with strong affinity for the variable region of an antibody kappa light chain
- Broad antibody fragment capture, suitable for formats such as Fabs, single-chain variable fragments and domain antibodies when the target contains a kappa light chain
- Rigid high-flow agarose matrix, designed to support productivity, process economy and process design flexibility
- 85 µm average particle size, providing a defined BioProcess resin format for packed-bed chromatography
- Approximate Fab binding capacity, about 25 mg Fab/mL medium at 10% breakthrough under stated test conditions
- High maximum flow velocity, up to 500 cm/h at 20 cm bed height under stated conditions
- Defined pH and cleaning conditions, with pH 2 to 10 working range and 15 mM NaOH recommended for cleaning-in-place
Benefits
- Helps capture antibody fragments that contain a kappa light chain using a specific protein L affinity interaction.
- Supports purification of smaller antibody formats where protein A may not be the preferred capture option.
- Can reduce process time and resin consumption through high dynamic binding capacity for suitable targets.
- Provides a scalable bulk resin format for moving from development work into larger packed-column workflows.
Why Choose the Capto L Antibody Fragment Resin, 200 mL (17547802)?
Choose the Capto L Antibody Fragment Resin, 200 mL (17547802) when you need a protein L affinity resin for antibody fragment purification where the target contains a kappa light chain. John Morris Group supplies Cytiva chromatography consumables for Australian laboratories and can help compare Capto L with newer MabSelect VL resin options where higher binding capacity or alkaline stability is required.
