The Capto L Antibody Fragment Resin, 25 mL (17547801) is a protein L affinity chromatography resin for purification of antibodies and antibody fragments containing kappa light chains. It combines a rigid high-flow agarose matrix with a recombinant protein L ligand, supporting selective capture of fragments such as Fabs, scFv, and dAbs from laboratory to process-scale workflows.
Key Features
- 25 mL bulk resin format, supplied as Capto L resin for antibody fragment purification workflows
- Protein L affinity chemistry, binding to the variable region of antibody kappa light chains
- Broad antibody fragment capture, suitable for fragments such as Fabs, single-chain variable fragments, and domain antibodies
- Rigid high-flow agarose matrix, supporting productivity, process economy, and scale-up flexibility
- Recombinant protein L ligand, produced from E. coli and coupled using epoxy chemistry
- Average 85 µm particle size, based on median particle size of the cumulative volume distribution
- Useful dynamic binding capacity, approximately 25 mg Fab per mL medium under specified breakthrough conditions
- High maximum flow velocity, up to 500 cm/h at 20 cm bed height under specified operating conditions
Benefits
- Supports efficient capture of antibody fragments containing kappa light chains in a selective affinity chromatography step.
- Helps reduce process time and resin volume through high binding capacity and low ligand leakage.
- Supports purification from lab scale to process scale using a resin platform available in multiple prepacked formats.
- Helps reduce non-specific binding and host cell-derived impurities through a hydrophilic base matrix.
Why Choose the Capto L Antibody Fragment Resin, 25 mL (17547801)?
The Capto L Antibody Fragment Resin, 25 mL (17547801) is a suitable choice for laboratories purifying antibody fragments such as Fab, scFv, and dAb formats that contain kappa light chains. John Morris Group supplies Cytiva chromatography resins to Australian laboratories, helping users source verified consumables for affinity purification and downstream process development.
