The PreDictor RoboColumn Ni Sepharose excel 600 µL (29714916) is a prepacked miniaturised IMAC chromatography column format for high-throughput process development of his-tagged protein purification workflows. It is supplied as one row of eight 600 µL columns packed with Ni Sepharose excel affinity resin, with each 600 µL column using a 30.0 mm bed height and 59 mm column height.
Key Features
- 600 µL miniaturised column volume, with a 30.0 mm bed height for scale-down chromatography studies
- Prepacked Ni Sepharose excel IMAC format, designed for capture and purification of histidine-tagged proteins
- Suitable for samples containing nickel stripping agents, supporting his-tagged protein workflows where this condition is relevant
- One row of eight columns, supplied with the same chromatography resin for parallel experiment setup
- Automated HTPD workflow support, suitable for robotic liquid handling workstations used in parallel chromatographic separations
- Polypropylene column and filter materials, with a verified 7 to 12 µm filter pore size
- Defined operating range, with 4°C to 30°C working temperature, up to 8.0 bar maximum working pressure, and 16 to 1000 cm/h flow velocity
- 96-position array plate arrangement, allowing columns to be arranged individually for flexible experiment design
Benefits
- Helps screen chromatographic conditions for his-tagged protein purification while reducing sample consumption during early process development.
- Supports purification method development for histidine-tagged proteins secreted into eukaryotic cell culture supernatants.
- Allows parallel testing of binding, wash, and elution conditions in a structured high-throughput workflow.
- Provides an eight-column row format that can be arranged to suit different experiment layouts.
Why Choose the PreDictor RoboColumn Ni Sepharose excel 600 µL (29714916)?
The PreDictor RoboColumn Ni Sepharose excel 600 µL format gives laboratories a practical route for miniaturised IMAC process development using a prepacked affinity chromatography column format. John Morris Group supplies PreDictor chromatography consumables across Australia, helping research and bioprocess teams source verified product variants for high-throughput workflows.
